Seasonal freezing and thawing process occurs in the non-growing season in the alpine grassland on the Tibetan Plateau. However, it is still unclear that how warming would affect this process in the alpine zone. From October 2013 to April 2014, the research used minirhizotron directily and soil temperature indirectly to investigate the effects of warming on the freezing and thawing process. The results showed that, warming significently increased soil temperature at the depths of 5, 10 and 20 cm, and soil temperatue in the winter warming plots was 0.01-0.18^oC higher than that in the annual warming plots. Wamrming significantly decreased the depth of soil frozen layer in the frozen period and thawing period of winter spring, but had no effect on the the depth of soil frozen layer in the thawing period of fall-winter. It is due to warming-induced redution on soil miosture. Warming significantly reduced lasting days of frozen period and increased lasting days thawing period of winter-spring, and did not affect the lasting days of freezing period of fall-winter. Effects of winter warming on frozen soil depths and lasting days were much greater than annual warming. The reults suggest that the enhanced freezing and thawing circles with a warming trend may subsequently affect soil carbon (C) and nitrogen (N) in this region.